Biacore is a life science products company, based in sweden. In the case of larger particles 52 nm in diameter, the detection limit increases and. Aspects of sensor chip usage that are specific to individual instruments are described in the. Biacore 8k provides sensitivity and stability that enables the differentiation of tight binders with dissociation rate constants, k d, down to 106 s1.
Overview of biacore systems and their applications. We recommend to use biacore x for the complex interactions and initial screenings. Standard protocols protocols introduction protocols these protocols are compiled from the literature from biacore ab. Use this handbook together with the specific user documentation for your biacore system, which will describe how to implement the assay principles in the instrument and system software. It provides high quality, labelfree interaction analysis for a fraction of the cost of existing solutions. Biacore 3000 instrument handbook free ebook download as pdf file. Rightclick menus insert chip and eject chip are used for docking and undocking the sensor chip respectively. Instrument engineers handbook, 4th edition, volume 1. Openspr is the worlds only benchtop surface plasmon resonance spr instrument. Biacore assay handbook ge healthcare life sciences. Align sensorgrams to chosen points in the x and ydirections. With a single click, the compact designed equipment offers. Manual run provides interactive control of the instrument operation. If you are new with the instrument and the technology, please read following manuals before starting to plan your experiments, you will find a lot of usefull information especially in the sensor surface and assay handbooks.
Biacore t200 is a versatile system for highquality characterization of molecular interactions, from ions to viruses. Biacore 8k is a highthroughput, highsensitivity spr system that enables efficient screening characterization, process optimization, and quality control of small molecules and biotherapeutics. Determine active concentrations of protein therapeutics and vaccines for inprocess control for batch release for qc biacore c key performance benefits use software wizards for fast assay development and sample analysis use a streamlined workflow with automatic result presentation biacore. Our unique nanostructured sensor surface uses localized spr lspr to deliver repeatable, highly sensitive kinetic data.
Biacore t200 instrument handbook, the instruction manual for operating the biacore t200 instrument. You can decide to make it visible only to you or to a restricted audience. Short essay on cities of future, crata theory essay. The biacore 4000 is ideal for laboratories that focus on drug discovery and antibody analysis with the ability to perform 60 hours of unattended operation, the biacore 4000 reduces labor by the researcher and increases productivity. Evaluation of the biacore 2000 instrument for screening of. Each user is required to use their own sensor chip for their experiments and these may either be purchased from biacore directly or from the facility. One platform for comprehensive characterization of molecular interactions in terms of kinetics, affinity, specificity, comparability, concentration, immunogenicity, and thermodynamics. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. The worlds first spr instrument dedicated to rapid determination of concentration in gxp environments. Get access to valuable additional material, such as detailed product information, support tools and more. Each angle of the reflected beam strikes the instruments detector at a different point and, thus, the detector continuously records the position of reduced light intensity and calculates the spr angle from that figure. Instrument engineers handbook, 4th edition, volume 1process measurement and analysis process variable and safety detectors, analyzers. More details are given in chapter 3 of the biacore t100 instrument handbook.
A valuable reference for further study of control valves is the isa handbook of control valves, second edition, 1976. Biacore t200 getting started guide, which in conjunction with the getting started reagent kit and cm5 sensor chip, provides users a selfguided tutorial through the basic steps of a basic biacore experiment performed using aminecoupling chemistry. Here the various biacore instrument platforms and their primary uses, ranging from semiautomated systems designed for simple, flexible basic research to fully automated, highthroughput systems, and systems designed to function in regulated environments, are all highlighted. This edition of the biacore x100 handbook applies to systems running software version 2. The handbook also includes a system description, safety instructions, specifications and a brief description of the spr principle. Biacore t200 instrument handbook 28976863 biacore t200 software handbook 28976878 biacore advisor tutorial, cdrom br100544 sensor surface handbook br100571 plastic vials, 7 mm package of vials br100212 plastic screw top vials and caps 10. This handbook describes biacore t100 control software and evaluation software, version 2. Single solution for interaction analysis allowing ranking, kinetics, affinity, concentration, and relative potency. The biacore t200 is an instrument for surface plasmon. Ge biacore t200 getting started guide ligand analyte. Safety information biacore x100 instrument contains mains voltage and handles liquids that may be hazardous.
Biacore t100 instrument handbook describes the instrumentation in the biacore t100 system, with instructions for operation, maintenance and troubleshooting. Its technology is often used to measure not only binding affinities, but kinetic rate constants and. Instrument engineers handbook for durco quarterturn. Factors influencing the detection limit of the lateral. Biacore maintenance kits are convenient kits for proper instrument maintenance. Evaluation of the biacore 2000 instrument for screening of low molecular weight affinity ligands title swedish abstract an important stage in the development of new affinity media for protein purification is the screening of potential affinity ligands. Sensor chips that are not docked in the instrument should be stored in closed containers. Scale to open a dialog box where fixed limits can be set for the x and yaxes. Data file 29050011 aa labelfree interaction analysis. These protocols apply to the biacore 20003000 instruments and software the following protocols are present. Biacore t200 getting started guide integrated structural biology. Schematic illustration showing the flow paths during loop injection. Ge healthcare labelfree interaction analysis biacore 8k.
Startup starting a biacore machine continue leaving an instrument for 34 days desorb cleaning the instrument once a week. Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. The biacore system, differential titration calorimetry dtc and a stoppedflow instrument have been used for measurement of physicochemical constants associated with. Since its first use in a realtime analysis of a biological system in 1990s, surface plasmon resonance spr has become an important optical biosensing technology in the areas of biochemistry, biology, and medical sciences because of its realtime, labelfree, and noninvasive nature. Biacore x ge healthcare is manual surface plasmon resonance based refractometer, appropriate for the introductory investigation of interactions with unknown properties. Biacore t100 software version 2 university of oxford. Dock the sensor chip in the instrument as described in the instrument handbook. Make surethe instrument is maintained as described in the instrument handbook. The instrument also features a rack hotel in which up to 10 rack trays can be loaded with automatic rack exchange. Biacore t200, ge healthcare, 28979415, edition ab 20.
The symbols o and represent open and closed valves respectively. Protein concentration, surface plasmon resonance spr, biacore, purification ge healthcare, biacore concentration analysis. Biacore 8k cytiva, formerly ge healthcare life sciences. If thisbehavior is observed, clean the flow system thoroughly as instructed in theinstrument handbook, then allow the instrument to equilibrate with a low flowof running buffer at least overnight. Biacore t200 getting started 28984098 edition ab 5 biacore t200 getting started biacore t200 getting started introduction this getting started handbook is designed as a selfstudy guide to introduce you to the basic operations of biacoretm t200, biacore t200 control software and biacore t200 evaluation software.
Manual run provides interactive contro1 of the instrument operation, executing commands sing1y as they are issued. Commercial spr devices are prohibitively expensive and require consumable sensor. Instrument procedures handbook iph share on facebook. Any functionality that is added in optional addon modules is described in separate documentation. Latest software updates and software notes software upgrade packages and additional licences software wizard templates to help speed up assay optimization and routine analyses. Biacore t200 for high sensitivity, labelfree interaction analysis. Parallel setup maximizing operational efficiency biacore 8k is designed to maximize operational efficiency by combining rapid highquality data with the smooth operation. The instrument is biacore t100 software handbook br100648 edition ae. Cross new frontiers in labelfree interaction analysis with biacore t200, ge healthcare, 28979412, edition aa 2010 data file. Biacore sensor surface handbook br100571 edition ab. Biacore t200 instrument handbook describes the instrumentation in the. Biacore x100 is intended for research use only and should not be used for diagnostic purposes in any clinical or in vitro procedures. The available sensor chip surfaces and immobilization techniques are.
In biacore instruments, monochromatic light in the shape of a wedge a broad distribution of incident angles is used. The chip with two flow cells is being used and all the reagents are injected manually. Biacore 3000 instrument handbook br100381 edition ag. Biacore t200 cytiva, formerly ge healthcare life sciences. Ge healthcare life sciences biacore t200 instrument handbook contents 1 introduction 1. The detection limit decreases from 80 to 3 ngml for antibodies with k d 1. Download, print, or view the complete iph pdf front cover pdf front page pdf table of contents pdf summary of changes pdf chapter.